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negative control on-targetplus non-targeting control pool  (Thermo Fisher)


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    Thermo Fisher negative control on-targetplus non-targeting control pool
    Negative Control On Targetplus Non Targeting Control Pool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/on-targetplus+non-targeting+control+pool/negative+control+on+targetplus+non+targeting+control+pool/pmc10034071-55-16-20
    Average 90 stars, based on 1 article reviews
    negative control on-targetplus non-targeting control pool - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Small Interfering RNA:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.

    Control:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.

    Transfection:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.

    Knockdown:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.

    Negative Control:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.

    Concentration Assay:

    Article Title: Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.
    Article Snippet: 215 216 Small interfering RNA (siRNA) transfection 217 The transient knockdown assays were performed with an ON-TARGETplus Non-targeting 218 Control Pool or separate ON-TARGETplus SMART pools targeting ALK2 (ACVR1), ALK3 219 (BMPR1A), ALK6 (BMPR1B) or SMAD4 (Thermo Fisher Scientific).

    Article Title: Activin A, B and AB decrease progesterone production by down-regulating StAR in human granulosa cells.
    Article Snippet: Activins are homoor heterodimers of inhibin β subunits that play important roles in the reproductive system.. Our previous work has shown that activins A (βAβA), B (βBβB) and AB (βAβB) induce aromatase/ estradiol, but suppress StAR/progesterone production in human granulosa-lutein cells.. However, the underlying molecular determinants of these effects have not been examined.

    Article Title: Modulation of glucocorticoid resistance in pediatric T-cell Acute Lymphoblastic Leukemia by increasing BIM expression with the PI3K/mTOR inhibitor BEZ235
    Article Snippet: ON-TARGETplus AKT1 SmartPool siRNA and ON-TARGETplus Non-Targeting Control Pool were purchased from Thermo-Scientific.

    Article Title: Canonical and Novel Non-Canonical Cholinergic Agonists Inhibit ATP-Induced Release of Monocytic Interleukin-1β via Different Combinations of Nicotinic Acetylcholine Receptor Subunits α7, α9 and α10
    Article Snippet: Cells were transfected with ON-TARGETplus human CHRNA7 (α7), CHRNA9 (α9) or CHRNA10 (α10) SMARTpool siRNA (Thermo Fisher Scientific, Schwerte, Germany) or with ON-TARGETplus non-targeting control pool (Thermo Fisher Scientific) as a negative control.

    Article Title: HIF-1α is essential for the augmentation of myometrial contractility during labor
    Article Snippet: ON-TARGETplus Human Hif1a (Hypoxia Inducible Factor 1 Subunit Alpha) small interfering RNA (siRNA) smartpool (L-004018-00-0005), ON-TARGETplus Non-targeting Control Pool (D-001810-10-05) (Thermo, USA).

    Article Title: Growth differentiation factor 8 suppresses cell proliferation by up-regulating CTGF expression in human granulosa cells.
    Article Snippet: Connective tissue growth factor (CTGF) is a matricellular protein that plays a critical role in the development of ovarian follicles.. Growth differentiation factor 8 (GDF8) is mainly, but not exclusively, expressed in the mammalian musculoskeletal system and is a potent negative regulator of skeletal muscle growth.. The aim of this study was to investigate the effects of GDF8 and CTGF on the regulation of cell proliferation in human granulosa cells and to examine its underlying molecular determinants.

    Article Title: Connective tissue growth factor mediates growth differentiation factor 8-induced increase of lysyl oxidase activity in human granulosa-lutein cells.
    Article Snippet: Lysyl oxidase (LOX) is an essential enzyme for the stabilization of the extracellular matrix (ECM) and the subsequent follicle and oocyte maturation.. Currently, there is limited information pertaining to the regulation of LOX activity in human ovarian tissue.. Growth differentiation factor 8 (GDF8) is a unique member of the transforming growth factor-b superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions.

    Article Title: Cancer-associated DDX3X mutations drive stress granule assembly and impair global translation
    Article Snippet: For and , HEK293T cells were transfected with with 0.25 μg/well (4-well glass slide, Millipore) of control plasmid, or with the indicated EGFP-DDX3X fusion constructs for 24 h using FuGENE 6 as indicated by the company.



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    KEY RESOURCES TABLE

    Journal: Cell host & microbe

    Article Title: Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis

    doi: 10.1016/j.chom.2021.05.009

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Millipore Sigma Cat# 407293 Recombinant Murine TNF-α Peprotech Cat# 315–01A Fixation/Permeabilization Solution Kit BD Bioscience Cat# 554714 ON-TARGETplus Mouse Zbp1 siRNA Horizon Cat# L-048021–00-0020 ON-TARGETplus Non-targeting Control Pool Horizon Cat# D-001810–10-05 Proteinase K New England BioLabs Cat# P8107 RNase A Thermofisher Cat# EN0531 RNase T1 Thermofisher Cat# EN0541 Fixable Viability Stain 700 BD Horizon Cat# 564997 DNase I Thermofisher Cat# 90083 SYTOX™ Green Nucleic Acid Stain Invitrogen Cat# S7020 DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) Invitrogen Cat# D1306 INTERFERin transfection reagent Polyplus Cat# 409–10 MAXblock Blocking Medium Active Motif Cat# 15252 ProLong Gold Antifade Reagent Thermo Fisher Cat# {"type":"entrez-protein","attrs":{"text":"P36930","term_id":"1248281091","term_text":"P36930"}} P36930 Mouse Interferon Beta PBL Assay Science Cat# 12401–1 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7570 BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Cat# 21580 Dynabeads™ M-280 Sheep Anti-Mouse IgG Thermo Fisher Cat# 11202D Cells and Virus L929 (NCTC clone 929) ATCC ATCC® CCL-1 HeLa cells Gift from George Pavlakis, NCI Bert Jacobs SVEC4–10 derived EV and FLAG-ZBP1 https://doi.org/10.15252/embr.201743947 Jason Upton Vaccinia virus (VACV) https://doi.org/10.1073/pnas.0431131100 https://doi.org/10.1128/JVI.69.10.6605-6608 https://doi.org/10.1128/JVI.75.2.850-856 https://doi.org/10.1006/viro.1996 https://doi.org/10.1016/j.virol.2005.01.006 Bert Jacobs Monkey poxvirus BEI Resources (MPXV) 7–61 (WRAIR) Software and Algorithms Prism9 https://www.graphpad.com/ Version 9.1.0 (216) FlowJo Becton Dickinson & Company (BD) Version FlowJo 10.7.1 Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ Leica LAS X software Leica Microsystems https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ Other BD FACSymphony™ A3 Flow cytometer BD A3 Leica SP8 instrument confocal microscope Leica Microsystems SP8 Open in a separate window KEY RESOURCES TABLE Vaccinia virus infection results in production of cytoplasmic Z-RNA Zα domains of vaccinia E3 and host ZBP1 compete for Z-RNA ZBP1 senses Z-RNA and drives necroptosis unless prevented by vaccinia E3 Zα E3 complexed with dsRNA promotes Z-RNA formation

    Techniques: Produced, In Vitro, Generated, Recombinant, Staining, Transfection, Blocking Assay, Cell Viability Assay, Derivative Assay, Software, Flow Cytometry, Microscopy